7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes Answer : a) the same enzyme that leaves the donor gene...
6. In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be ... not the non-transformants can survive. (d) transformants cannot survive, but non-transformantscan not.
Name the enzymes associated with recombinant DNA technology and explain their roles in correct sequence.
12 Which of the following molecules forms lengths of DNA with ‘sticky ends’? a) DNA ligase b) DNA polymerase c) RNA polymerase d) Restriction enzyme Answer : ANS: d Restriction enzyme...
6) In agarose gel electrophoresis, DNA molecules are separated on the basis of their- a)charge only b)size only c)charge to size ratio d)all of the above Answer : d)all of the above...
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